Severe acute respiratory syndrome coronavirus accessory protein 9b is a virion-associated protein.
Identifieur interne : 002A20 ( Main/Exploration ); précédent : 002A19; suivant : 002A21Severe acute respiratory syndrome coronavirus accessory protein 9b is a virion-associated protein.
Auteurs : Ke Xu [République populaire de Chine] ; Bo-Jian Zheng ; Rong Zeng ; Wei Lu ; Yong-Ping Lin ; Liang Xue ; Li Li ; Lei-Lei Yang ; Chen Xu ; Jie Dai ; Fei Wang ; Qing Li ; Qing-Xi Dong ; Rui-Fu Yang ; Jia-Rui Wu ; Bing SunSource :
- Virology [ 1096-0341 ] ; 2009.
Descripteurs français
- KwdFr :
- ADN viral (génétique), ARN messager (), ARN messager (génétique), Animaux, Anticorps antiviraux (génétique), Anticorps antiviraux (métabolisme), Cadres ouverts de lecture (génétique), Cellules Vero, Lignée cellulaire, Plasmides, Protéines virales structurales (génétique), Protéines virales structurales (métabolisme), Souris, Virion (génétique), Virion (métabolisme), Virus du SRAS (génétique), Virus du SRAS (immunologie), Virus du SRAS (métabolisme).
- MESH :
- génétique : ADN viral, ARN messager, Anticorps antiviraux, Cadres ouverts de lecture, Protéines virales structurales, Virion, Virus du SRAS.
- immunologie : Virus du SRAS.
- métabolisme : Anticorps antiviraux, Protéines virales structurales, Virion, Virus du SRAS.
- ARN messager, Animaux, Cellules Vero, Lignée cellulaire, Plasmides, Souris.
English descriptors
- KwdEn :
- Animals, Antibodies, Viral (genetics), Antibodies, Viral (metabolism), Cell Line, Chlorocebus aethiops, DNA, Viral (genetics), Mice, Open Reading Frames (genetics), Plasmids, RNA, Messenger (chemistry), RNA, Messenger (genetics), SARS Virus (genetics), SARS Virus (immunology), SARS Virus (metabolism), Vero Cells, Viral Structural Proteins (genetics), Viral Structural Proteins (metabolism), Virion (genetics), Virion (metabolism).
- MESH :
- chemical , chemistry : RNA, Messenger.
- chemical , genetics : Antibodies, Viral, DNA, Viral, RNA, Messenger, Viral Structural Proteins.
- chemical , metabolism : Antibodies, Viral, Viral Structural Proteins.
- genetics : Open Reading Frames, SARS Virus, Virion.
- immunology : SARS Virus.
- metabolism : SARS Virus, Virion.
- Animals, Cell Line, Chlorocebus aethiops, Mice, Plasmids, Vero Cells.
Abstract
Eight accessory proteins have been identified in severe acute respiratory syndrome-associated coronavirus (SARS-CoV). They are believed to play roles in the viral life cycle and may contribute to the pathogenesis and virulence. ORF9b as one of these accessory proteins is located in subgenomic mRNA9 and encodes a 98 amino acid protein. However, whether 9b protein is a structural component of SARS-CoV particles remains unknown. In this study, we demonstrate that 9b protein is translated from bicistronic mRNA9 via leaky ribosome scanning and it is incorporated into both virus-like particles (VLPs) and purified SARS-CoV virions. Further analysis shows that sufficient incorporation of 9b protein into VLPs is dependent upon the co-expression of E and M proteins, but not upon the presence of either S or N protein. Our data indicate that 9b protein of SARS-CoV is another virion-associated accessory protein. This finding will lead to a better understanding of the properties of the SARS-CoV 9b protein.
Url:
DOI: 10.1016/j.virol.2009.03.032
PubMed: 19394665
Affiliations:
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Le document en format XML
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200025</wicri:noRegion></affiliation></author><author><name sortKey="Zheng, Bo Jian" sort="Zheng, Bo Jian" uniqKey="Zheng B" first="Bo-Jian" last="Zheng">Bo-Jian Zheng</name></author><author><name sortKey="Zeng, Rong" sort="Zeng, Rong" uniqKey="Zeng R" first="Rong" last="Zeng">Rong Zeng</name></author><author><name sortKey="Lu, Wei" sort="Lu, Wei" uniqKey="Lu W" first="Wei" last="Lu">Wei Lu</name></author><author><name sortKey="Lin, Yong Ping" sort="Lin, Yong Ping" uniqKey="Lin Y" first="Yong-Ping" last="Lin">Yong-Ping Lin</name></author><author><name sortKey="Xue, Liang" sort="Xue, Liang" uniqKey="Xue L" first="Liang" last="Xue">Liang Xue</name></author><author><name sortKey="Li, Li" sort="Li, Li" uniqKey="Li L" first="Li" last="Li">Li Li</name></author><author><name sortKey="Yang, Lei Lei" sort="Yang, Lei Lei" uniqKey="Yang L" first="Lei-Lei" last="Yang">Lei-Lei Yang</name></author><author><name sortKey="Xu, Chen" sort="Xu, Chen" uniqKey="Xu C" first="Chen" last="Xu">Chen Xu</name></author><author><name sortKey="Dai, Jie" sort="Dai, Jie" uniqKey="Dai J" first="Jie" last="Dai">Jie Dai</name></author><author><name sortKey="Wang, Fei" sort="Wang, Fei" uniqKey="Wang F" first="Fei" last="Wang">Fei Wang</name></author><author><name sortKey="Li, Qing" sort="Li, Qing" uniqKey="Li Q" first="Qing" last="Li">Qing Li</name></author><author><name sortKey="Dong, Qing Xi" sort="Dong, Qing Xi" uniqKey="Dong Q" first="Qing-Xi" last="Dong">Qing-Xi Dong</name></author><author><name sortKey="Yang, Rui Fu" sort="Yang, Rui Fu" uniqKey="Yang R" first="Rui-Fu" last="Yang">Rui-Fu Yang</name></author><author><name sortKey="Wu, Jia Rui" sort="Wu, Jia Rui" uniqKey="Wu J" first="Jia-Rui" last="Wu">Jia-Rui Wu</name></author><author><name sortKey="Sun, Bing" sort="Sun, Bing" uniqKey="Sun B" first="Bing" last="Sun">Bing Sun</name></author></analytic><series><title level="j">Virology</title><idno type="eISSN">1096-0341</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Viral (genetics)</term><term>Antibodies, Viral (metabolism)</term><term>Cell Line</term><term>Chlorocebus aethiops</term><term>DNA, Viral (genetics)</term><term>Mice</term><term>Open Reading Frames (genetics)</term><term>Plasmids</term><term>RNA, Messenger (chemistry)</term><term>RNA, Messenger (genetics)</term><term>SARS Virus (genetics)</term><term>SARS Virus (immunology)</term><term>SARS Virus (metabolism)</term><term>Vero Cells</term><term>Viral Structural Proteins (genetics)</term><term>Viral Structural Proteins (metabolism)</term><term>Virion (genetics)</term><term>Virion (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN viral (génétique)</term><term>ARN messager ()</term><term>ARN messager (génétique)</term><term>Animaux</term><term>Anticorps antiviraux (génétique)</term><term>Anticorps antiviraux (métabolisme)</term><term>Cadres ouverts de lecture (génétique)</term><term>Cellules Vero</term><term>Lignée cellulaire</term><term>Plasmides</term><term>Protéines virales structurales (génétique)</term><term>Protéines virales structurales (métabolisme)</term><term>Souris</term><term>Virion (génétique)</term><term>Virion (métabolisme)</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (immunologie)</term><term>Virus du SRAS (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>RNA, Messenger</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Antibodies, Viral</term><term>DNA, Viral</term><term>RNA, Messenger</term><term>Viral Structural Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Antibodies, Viral</term><term>Viral Structural Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Open Reading Frames</term><term>SARS Virus</term><term>Virion</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN viral</term><term>ARN messager</term><term>Anticorps antiviraux</term><term>Cadres ouverts de lecture</term><term>Protéines virales structurales</term><term>Virion</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>SARS Virus</term><term>Virion</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Anticorps antiviraux</term><term>Protéines virales structurales</term><term>Virion</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Chlorocebus aethiops</term><term>Mice</term><term>Plasmids</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ARN messager</term><term>Animaux</term><term>Cellules Vero</term><term>Lignée cellulaire</term><term>Plasmides</term><term>Souris</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Eight accessory proteins have been identified in severe acute respiratory syndrome-associated coronavirus (SARS-CoV). They are believed to play roles in the viral life cycle and may contribute to the pathogenesis and virulence. ORF9b as one of these accessory proteins is located in subgenomic mRNA9 and encodes a 98 amino acid protein. However, whether 9b protein is a structural component of SARS-CoV particles remains unknown. In this study, we demonstrate that 9b protein is translated from bicistronic mRNA9 via leaky ribosome scanning and it is incorporated into both virus-like particles (VLPs) and purified SARS-CoV virions. Further analysis shows that sufficient incorporation of 9b protein into VLPs is dependent upon the co-expression of E and M proteins, but not upon the presence of either S or N protein. Our data indicate that 9b protein of SARS-CoV is another virion-associated accessory protein. This finding will lead to a better understanding of the properties of the SARS-CoV 9b protein.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Dai, Jie" sort="Dai, Jie" uniqKey="Dai J" first="Jie" last="Dai">Jie Dai</name><name sortKey="Dong, Qing Xi" sort="Dong, Qing Xi" uniqKey="Dong Q" first="Qing-Xi" last="Dong">Qing-Xi Dong</name><name sortKey="Li, Li" sort="Li, Li" uniqKey="Li L" first="Li" last="Li">Li Li</name><name sortKey="Li, Qing" sort="Li, Qing" uniqKey="Li Q" first="Qing" last="Li">Qing Li</name><name sortKey="Lin, Yong Ping" sort="Lin, Yong Ping" uniqKey="Lin Y" first="Yong-Ping" last="Lin">Yong-Ping Lin</name><name sortKey="Lu, Wei" sort="Lu, Wei" uniqKey="Lu W" first="Wei" last="Lu">Wei Lu</name><name sortKey="Sun, Bing" sort="Sun, Bing" uniqKey="Sun B" first="Bing" last="Sun">Bing Sun</name><name sortKey="Wang, Fei" sort="Wang, Fei" uniqKey="Wang F" first="Fei" last="Wang">Fei Wang</name><name sortKey="Wu, Jia Rui" sort="Wu, Jia Rui" uniqKey="Wu J" first="Jia-Rui" last="Wu">Jia-Rui Wu</name><name sortKey="Xu, Chen" sort="Xu, Chen" uniqKey="Xu C" first="Chen" last="Xu">Chen Xu</name><name sortKey="Xue, Liang" sort="Xue, Liang" uniqKey="Xue L" first="Liang" last="Xue">Liang Xue</name><name sortKey="Yang, Lei Lei" sort="Yang, Lei Lei" uniqKey="Yang L" first="Lei-Lei" last="Yang">Lei-Lei Yang</name><name sortKey="Yang, Rui Fu" sort="Yang, Rui Fu" uniqKey="Yang R" first="Rui-Fu" last="Yang">Rui-Fu Yang</name><name sortKey="Zeng, Rong" sort="Zeng, Rong" uniqKey="Zeng R" first="Rong" last="Zeng">Rong Zeng</name><name sortKey="Zheng, Bo Jian" sort="Zheng, Bo Jian" uniqKey="Zheng B" first="Bo-Jian" last="Zheng">Bo-Jian Zheng</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Xu, Ke" sort="Xu, Ke" uniqKey="Xu K" first="Ke" last="Xu">Ke Xu</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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